Biomarkers

The quality assurance programme for harmonisation of biomarker responses in aquatic species is led by the Norwegian Institute for Water Research (NIVA), Norway. The main component of the programme involves an inter-calibration using prepared standard samples.

Selected biomarkers for 2007-2008

Ethoxyresorufin O-deethylase (EROD) activity + Protein measurement Cytochrome P450 1A (CYP1A) concentration - ELISA (optional) EROD and CYP1A are both are sensitive and commonly used biomarkers for planar polychlorinated biphenyls (PCBs), polyaromatic hydrocarbons (PAHs) and dioxins that are capable of binding to and activating the arylhydrocarbon (Ah) receptor. EROD and CYP 1A activity will be measured in the microsomal fraction of liver homogenates of fish from both field samples and samples from laboratory exposures. CYP1A ELISA is an immunoassay that measures the concentration of CYP1A proteins, whereas EROD activity is the enzymatic activity of the CYP1A protein.

Protein measurement is used to normalise data from various biomarker analyses to total protein content of the sample and proved necessary for proper inter-assay comparison of results. The protein measurement involves the use of a protein colouring dye that may be measured spectrophometrically and related to the colouring of a known protein solution.

Ring test information and Registration Fees

All intercalibrations will be performed with samples from dab (Limanda limanda) and Atlantic cod (Gadus morhua).

Information for participants

A range of CYP1A antibodies are available from Biosense (http://www.biosense.com); we recommend CP-226.

Note: BEQUALM has not evaluated the products developed, manufactured, and marketed by the above company, nor does it provide any warranty or assurance as to the accuracy or suitability of the products. Listing should not be taken as endorsement of any kind. BEQUALM is not responsible for the contents or reliability of the linked web sites.

Ring test package

The ring test package includes the shipment of pre-prepared reference material, technical support during assay performance period, validation and reporting of the assay results. The report will include the results from all ring test participants (coded to preserve anonymity), and a comparison of performance of the participating laboratories. Protocols for the individual assays may be supplied to participants on demand.

Provisional Timetable of Events

To participate please contact bequalm@niva.no