Micronuclei Formation

The frequency of micronuclei (MN) in mussel haemocytes and fish erythrocytes is regarded as an important tool for in-situ monitoring of genotoxicity.

The MN are chromatin-containing structures that are surrounded by a membrane and have no detectable link to the cell nucleus. As an index of chromosomal damage, the MN assay is based on the enumeration of downstream aberrations after DNA damage and reveals a time-integrated response to complex mixtures of pollutants.

A preliminary intercalibration was conducted in 2018-2019 to investigate and optimise the incorporation of MN intercalibrations into the broader BEQUALM programme. Over 150 photographs of stained haemocytes were distributed to the participating laboratories for assessment of 1) micronuclei formation, 2) nuclear buds and 3) bi-nucleated cells.

Forthcoming Intercalibrations

There are no intercalibrations confirmed for this period, although plans are currently being prepared. Please check back for more information.